Клонування і бактеріальна експресія цитокінподібного некаталітичного домена тирозил-тРНК синтетази бика
DOI:
https://doi.org/10.7124/bc.0004EAАнотація
В бактеріальній системі експресії у векторі рЕТ15b кланован та експресовано цитокінподібний С-кінцевий не каталітичний домен тирозил-тРНК синтетази бика (D322 S328). З використанням метал-хелатної хроматографії на Ni-NTA-агарозі виділено рекомбінантний білок у гомогенному стані за даними гель-електрофорезу.Посилання
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