The aclion of vitamin E on RNA- and DNA-polymerase activity of rat liver mitochondria
DOI:
https://doi.org/10.7124/bc.000489Abstract
The action of tocopherol on RNA- and DNA-polymerase activity of isolated rat liver mitochondria in condition of its joined addition with Ca2+ -ionophor A23187, Ca2+-channel blacker verapamil or protein kinase C inhibitor pliorbol I2-myristate-13-acetate (PMA) were studied. It was found, that tocopherol, A23187 and verapamil effected RNA and DNA synthesis in mitochondria only if added together with fraction of tocopherol-binding proteins. In case of addition of this fraction together with tocopherol and A23J87 or verapamil, the RNA-polymerase activity of mitochondria of normal rats increased compared to assayes in which only tocopherol and tocoplierot-binding proteins were added. Unlike this, joint addition of tocopherol binding protein, A23187 and verapamil caused inhibition of DNA synthesis in mitochondria of normal but not vitamin E-deficient ruts. The addition of tocopherol decreased the action of these compounds. There were found the differences between RNA-polymerase activity of normal and vitamin E-deficient rats in presence of PMA. It was suggested that Ca ions and protein kinase C may be involved in action of vitamin E on RNA synthesis in rat liver mitochondria.References
Kapralov AA, Petrova GV, Donchenko GV. Physico-chemical properties and biological role of alpha-tocopherol binding proteins. Usp Sovrem Biol. 1993; 113(3):313-26.
Rozen VB. [A superfamily of nuclear hormone receptors--adaptive transcription factors]. Biokhimiia. 1991;56(3):565-71.
Donchenko GV. Ubiquinone biochemistry. Kiev: Naukova Dumka, 1988. 240 p.
Minchenko AG, Dudareva NA. Mitochondrial genome. Novosibirsk: Nauka, 1990. 194 p.
Petrova GV, Kapralov AA, Kuzmenko IV, Donchenko GV Investigation of tocopherol-binding proteins in membranes of the rat liver mitochondria. Ukr Biokhim Zh. 1990;62(2):29-35.
Petrova GV, Kapralov AA, Izhokina IA, Donchenko GV. [Effect of alpha-tocopherol and ubiquinone on mitochondrial RNA polymerase activity. The role of tocopherol-binding proteins]. Biokhimiia. 1994;59(4):575-81.
Boscoboinik D, Szewczyk A, Hensey C, Azzi A. Inhibition of cell proliferation by alpha-tocopherol. Role of protein kinase C. J Biol Chem. 1991;266(10):6188-94.
Pal'mina NP, Mal'tseva EL, Kurnakova NV, Burlakova EB. [The effect of alpha-tocopherol over a wide range of concentrations (10(-2)-10(-17) M) on protein kinase C activity. Connection with proliferation and tumor growth]. Biokhimiia. 1994;59(2):193-200.
Kleineke J, S?ling HD. Mitochondrial and extramitochondrial Ca2+ pools in the perfused rat liver. Mitochondria are not the origin of calcium mobilized by vasopressin. J Biol Chem. 1985;260(2):1040-5.
Malviya AN, Block C. Nuclear protein kinase C and signal transduction. Receptor. 1993 Winter;3(4):257-75.
Woodgett JR, Hunter T, Gould KL. Protein kinase C and its role in cell growth. Cell Membrane. Methods and reviews. Eds E. L. Elson, W. A. Frasier, R. Glaser-New York: Plenum Publ. Corp., 1987. Vol. 3: 1-88.
Levitsky EL, Gubskiy JuI. Regulatory effect of calcium ions and cyclic nucleotides on DNA synthesis in mammalian cells. Biokhimiya zhivotnykh i cheloveka. 1990; 14: 33-44.
Almazov A, Shlyahto EV. Ca antagonists and their use in hypertension. Intern Med Reviews. 1993; 1(4): 305-309.
Avdonin PV, Tkachuk VA. Intracellular calcium receptors. M.: Nauka, 1994. 288 p.
Parsons DF, Williams GR. Isolation and purification of the outer membrane and inner membrane of liver mitochondria. Methods Enzymol. 1967;443–8.
Transcription and translation - a practical approach: Edited by B D Hames and S J Higgins. IRL Press, Oxford. 1984. 328 p.
Donchenko GY, Kapralov AA, Petrova GY. Alfa-tocopherol prevents the inhibitory effects of A23187 and verapamil on RNA synthesis in isolated rat liver nuclei: Xllth Int. Congr. of Pharmacol. (July 1994). Can J Physiol Pharmacol. 1994; 72(1): 247.
Maguire JJ, Wilson DS, Packer L. Mitochondrial electron transport-linked tocopheroxyl radical reduction. J Biol Chem. 1989;264(36):21462-5.
Frei B, Kim MC, Ames BN. Ubiquinol-10 is an effective lipid-soluble antioxidant at physiological concentrations. Proc Natl Acad Sci U S A. 1990;87(12):4879-83.
Aronica SM, Kraus WL, Katzenellenbogen BS. Estrogen action via the cAMP signaling pathway: stimulation of adenylate cyclase and cAMP-regulated gene transcription. Proc Natl Acad Sci U S A. 1994;91(18):8517-21. ttp://
Moyer ML, Borror KC, Bona BJ, DeFranco DB, Nordeen SK. Modulation of cell signaling pathways can enhance or impair glucocorticoid-induced gene expression without altering the state of receptor phosphorylation. J Biol Chem. 1993;268(30):22933-40.
Nemere I, Zhou LX, Norman AW. Nontranscriptional effects of steroid hormones. Receptor. 1993 Winter;3(4):277-91.
Kashkarov KP, Vasil'eva EV, Ruuge EK. [Generation of superoxide radicals by the mitochondrial respiratory chain of isolated cardiomyocytes]. Biokhimiia. 1994;59(6):813-8.
Shoji Y, Uedono Y, Ishikura H, Takeyama N, Tanaka T. DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation. Immunology. 1995;84(4):543-8.
Kristal BS, Chen J, Yu BP. Sensitivity of mitochondrial transcription to different free radical species. Free Radic Biol Med. 1994;16(3):323–9.
