The use of polyamines for creation of effective reconstructed protein synthesizing system from rabbit liver
DOI:
https://doi.org/10.7124/bc.000461Abstract
A cell-free reconstructed protein-synthesizing system from rabbit liver has been developed. The system contains highly purified homologous components of translation apparatus and is characterized by a sufficient rate of polypeptide synthesis. The use of spermine and spermidine has allowed to optimize the activity of the system at physiological concentration of Mg2+.References
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