Characterization of catalytic activity in vitro of the ribozyme targeted to tat-RNA of HIV-I
DOI:
https://doi.org/10.7124/bc.000457Abstract
Essential properties of catalytic activity in vitro of the anti-tat RNA (HIV-I) hammerhead ribozyme have been studied. Spermidine-dependencies, discovered by us earlier, has supported. Optimal concentration of magnesium ion were established and was equal 5–10 mM. Temperature optimum of reaction was 30 °C. 20 % formamide has stimulating influence on efficiency of the cleavage at 15 °C. Time-dependence course of efficiency of reaction also were carried out.References
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