A proteolytic biosensor based on a pH-sensititive field effect transistor. II. Investigation of operation in model solutions

Authors

  • O. A. Beloivan Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. P. Soldatkin Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • N. F. Starodub Palladin Institute of Biochemistry, NAS of Ukraine 9, Leontovycha Str., Kyiv, Ukraine, 01601 Author
  • A. V. El'skaya Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000436

Abstract

The laboratory prototype of biosensor has been fabricated on the basis of trypsin immobilized on the gate surface of pH-sensitive field effect transistor which should be useful for detection of the total protein concentration in biological fluids. The optimal conditions to provide marswements have been determined. The characteristics of its operation stability and accuracy in a model system have been obtained. The biosensor prototype can measure concentrations of Na-benzoil-arginin ethyl ester in the range of 0.2-3.0 mM. The effects of pH, buffer capacity and ionic strength on the response of the biosensor have been investigated. All the results have been discussed in frame of the problem of detecting total protein concentration in biological fluids.

References

Fu H, Anzai J, Osa T, Matsuo T. Proteolytic enzyme sensors using an ion-sensitive field effect transistor. Chem Pharm Bull (Tokyo). 1988;36(3):1190-3.

Shul’ga AA, Strikha VI, Soldatkin AP, El’skaya AV, Maupas H, Martelet C, et al. Removing the influence of buffer concentration on the response of enzyme field effect transistors by using additional membranes. Anal Chim Acta. 1993;278(2):233–6.

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Published

1996-07-20

Issue

Vol. 12 No. 4 (1996)

Section

Biopolymers and Cell