Isolation and amino acid composition of peptides released by means of cleavage of Penicillium vitale catalase by the Staphylococcus proteinase

Authors

  • N. V. Latyschko Palladin Institute of Biochemistry, NAS of Ukraine 9, Leontovycha Str., Kyiv, Ukraine, 01601 Author
  • T. L. Levitina Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • O. S. Miroschnichenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • L. V. Gudkova Palladin Institute of Biochemistry, NAS of Ukraine 9, Leontovycha Str., Kyiv, Ukraine, 01601 Author
  • E. A. Kozlov Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.00035A

Abstract

38 peptides containing 578 amino acid residues were isolated by gel-filtration, ion-exchange chromatography and paper highe-voltage electrophoresis and chromotagraphy.

References

Kozlov EA, Gudkova LV, Levitina TL, Kirilenko MT, Miroshnichenko OS. Additional investigation of Penicillium vitale catalase tryptic peptides. Biopolym Cell. 1993; 9(1):22-5.

Levitina TL, Bobrovskaja MT, Gusak NM, Miroschnichenko OS, Gudkova LV, Latyschko NV, Kozlov EA. Tryptic peptides of Penicillium vitale maleyl-catalase. 2. The structure peptides. Biopolym Cell. 1993; 9(3):42-5.

Levitina TL, Gusak NM, Rodnin NV, Kirilenko MT, Miroshnichenko OS, Atepalikhina SA, Gudkova LV, Kozlov EA. Cyanogen bromide fragments of Penicillium vitale catalase. Biopolym Cell. 1989; 5(5):55-63.

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Kozlov EA, Levitina TL, Katsman MS, Gusak NM, Ovander MN, Serebryany SB. Tryptic fragments of the maleylated inclusion body protein of the silkworm nuclear polyhedrosis virus. II. Amino acid sequence of the fragments. Russian Journal of Bioorganic Chemistry. 1978; 4(8):1036-1047.

Gray WR. Sequence degradation plus dansylation. Methods Enzymol. 1967; 11:469-475.

Vainshtein BK, Melik-Adamyan WR, Barynin VV, Vagin AA, Grebenko AI, Borisov VV, Bartels KS, Fita I, Rossmann MG. Three-dimensional structure of catalase from Penicillium vitale at 2.0 A resolution. J Mol Biol. 1986;188(1):49-61.

Published

1993-05-20

Issue

Section

Structure and Function of Biopolymers