Fragment K 2-3 of plasminogen molecule carries a lysine-binding site
DOI:
https://doi.org/10.7124/bc.000104Abstract
Limited pepsin digestion of plasminogen fragment K 1—3 leads to the formation of the first kringle and structure which contains the second and third kringles (fragment K 2—3). The isolated fragments are characterized according to molecular masses and amino acid composition. Fragment K 2—3 is shown to be able of binding with lysine-Sepharose, which indicates the existence of a lysine-binding site in its structure.References
Colleti D. On the regulation and control of fibrinolysis. Thromb Haemostas. 1980; 43(1):77-89.
Wiman B. Primary structure of the B-chain of human plasmin. Eur J Biochem. 1977;76(1):129-37.
Wiman B, Wallen P. Activation of human plasminogen by an insoluble derivative of urokinase. Structural changes of plasminogen in the course of activation to plasmin and demonstration of a possible intermediate compound. Eur J Biochem. 1973;36(1):25-31.
Sottrup-Jensen L, Clayes H, Zajdel M et al. The primary structure of human plasminogen : isolation of two lysine-binding fragments and one "Mini-plasminogen" (M. W. 38000) by elastase catalyzed specific limited proteolysis. Progress in chemical fibrinolysis and thrombolysis. Eds. J. F. Davidson, R. H. Roman, M. M. Samama, P. S. Desnoyers. New York : Raven press, 1978, v. 3:191-209.
Castellino FJ. Recent advances in the chemistry of the fibrinolytic system. Chem Rev. 1981; 81(5):431-46.
Wiman B, Collen D. Molecular mechanism of physiological fibrinolysis. Nature. 1978;272(5653):549-50.
Wiman B, Wallen P. The specific interaction between plasminogen and fibrin. A physiological role of the lysine binding site in plasminogen. Thromb Res. 1977;10(2):213-22.
Trexler M, Vali Z, Patthy L. Structure of the omega-aminocarboxylic acid-binding sites of human plasminogen. Arginine 70 and aspartic acid 56 are essential for binding of ligand by kringle 4. J Biol Chem. 1982;257(13):7401-6.
Novokhatny VV, Kudinov SA, Privalov PL. Domains in human plasminogen. J Mol Biol. 1984;179(2):215-32.
Deutsch DG, Mertz ET. Plasminogen: purification from human plasma by affinity chromatography. Science. 1970;170(3962):1095-6.
Cummins P, Perry SV. The subunits and biological activity of polymorphic forms of tropomyosin. Biochem J. 1973;133(4):765-77.
Novokhatniy VV, Kudinov SA. Getting miniplazminogena and pieces 1-4 peptic proteolysis of human plasminogen. Dokl Akad Nauk SSSR. 1984; )5):62-5.
