DNA import competence and mitochondrial genetics

Authors

  • F. Weber-Lotfi Institute of Plant Molecular Biology, CNRS and University of Strasbourg (UdS) 12, General Zimmer Str., Strasbourg, France, 67084 Author
  • D. V. Mileshina Institute of Plant Molecular Biology, CNRS and University of Strasbourg (UdS) 12, General Zimmer Str., Strasbourg, France, 67084; Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences 132, Lermontova Str., Irkutsk, Russian Federation, 664033 Author
  • N. Ibrahim Institute of Plant Molecular Biology, CNRS and University of Strasbourg (UdS) 12, General Zimmer Str., Strasbourg, France, 67084; Institute for Cell and Molecular Biosciences, Medical School, Newcastle University Framlington Place, Newcastle upon Tyne, UK, NE2 4HH Author
  • M. V. Koulintchenko Institute of Plant Molecular Biology, CNRS and University of Strasbourg (UdS) 12, General Zimmer Str., Strasbourg, France, 67084; Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences 132, Lermontova Str., Irkutsk, Russian Federation, 664033; Institute for Cell and Molecular Biosciences, Medical School, Newcastle University Framlington Place, Newcastle upon Tyne, UK, NE2 4HH Author
  • G. G. M. D'Souza School of Pharmacy - Boston, MCPHS University 179, Longwood Ave., Boston, USA, MA 02115 Author
  • V. Saxena School of Pharmacy - Boston, MCPHS University 179, Longwood Ave., Boston, USA, MA 02115 Author
  • Yu. M. Konstantinov Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences 132, Lermontova Str., Irkutsk, Russian Federation, 664033 Author
  • R. N. Lightowlers Institute for Cell and Molecular Biosciences, Medical School, Newcastle University Framlington Place, Newcastle upon Tyne, UK, NE2 4HH Author
  • A. Dietrich Institute of Plant Molecular Biology, CNRS and University of Strasbourg (UdS) 12, General Zimmer Str., Strasbourg, France, 67084 Author

DOI:

https://doi.org/10.7124/bc.000881

Keywords:

mitochondria, DNA import, mitochondrial transfection, plant, <em>Saccharomyces cerevisiae</em>, mammal

Abstract

Aim. To understand the mechanism(s) underlying mitochondrial competence for DNA uptake and to exploit these pathways for the development of in vivo models of gene therapy. Methods. DNA uptake into isolated mitochondria from plant or from mutant Saccharomyces cerevisiae defective for mitochondrial proteins and carriers, biochemical approaches and transfection of mammalian cells with DNA bound to mitochondriotropic liposomes. Results. Special focus on the inner membrane showed the involvement of isoforms of the adenine nucleotide translocator and the contribution of proteins controlling mitochondrial morphology in DNA uptake into yeast organelles. Transfection assays led to significant incorporation of a mitochondrial construct into mammalian cells and expression of a marker gene. Conclusions. The data imply that there are multiple mitochondrial DNA import pathways. On the other hand, preliminary results suggest that mitochondriotropic liposomes can deliver DNA to mitochondria in live mammalian cells.

References

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Published

2014-01-20

Issue

Vol. 30 No. 1 (2014)

Section

Short Communications