Structure and function of oncogene-transfected immortal cells
DOI:
https://doi.org/10.7124/bc.00087CKeywords:
oncogene CHI3L1, atomic force microscopy, reactive oxygen species (ROS), 293 cells (HEK293), combinatorial chemotherapy, chromosome instability (CIN).Abstract
Previously we have characterized a new oncogene CHI3L1, overexpressed in the glioblastoma and the malignant 293_CHI3L1 cells, stably producing the CHI3L1 angiogenic oncoprotein. The 293_CHI3L1 cells proliferated faster and acquired a higher ability for anchorage-independent growth. Here, we report the atomic force microscopy data and functional characteristics of these cells. The constitutive CHI3L1 expression leads to the increased resistance to the damages by oxidative substances and promotes the chromosome instability in the 293 cells. According to the data of the last clinical investigations, anti-cancer therapy should be targeted not at the individual genes, but at the pathological effects they caused. We propose a complex treatment of gliomas including multi-target inhibitors, which can be delivered to the brain tumor by a specific nanoparticle vector.References
Garifulin OM, Shostak KO, Dmitrenko VV, Rozumenko VD, Khomenko OV, Zozulya YuP, Zehetner G, Kavsan VM. The genes SOX-2 and HC gp-39 are overexpressed in astrocytic gliomas. Biopolym Cell. 2002; 18(4):324–9.
Kavsan VM, Baklaushev VP, Balynska OV, Iershov AV, Areshkov PO, Yusubalieva GM, Grinenko NP, Victorov IV, Rymar VI, Sanson M, Chekhonin VP. Gene encoding chitinase 3-like 1 protein (CHI3L1) is a putative oncogene. Int J Biomed Sci. 2011; 7(3):230–7.
Graham FL, Smiley J, Russell WC, Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977; 36(1):59–74.
Shaw G, Morse S, Ararat M, Graham FL. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 2002; 16(8):869–71.
Watson J. Oxidants, antioxidants and the current incurability of metastatic cancers. Open Biol. 2013; 3(1):120144.
Duesberg P, Mandrioli D, McCormack A, Nicholson JM. Is carcinogenesis a form of speciation? Cell Cycle. 2011; 10 (13): 2100–14.
Vincent MD. Cancer: beyond speciation. Adv Cancer Res. 2011; 112:283–350.
Stepanenko AA, Kavsan VM. Evolutionary karyotypic theory of cancer versus conventional cancer gene mutation theory. Biopolym Cell. 2012; 28(4):267–80.
Ljubimova JY, Fujita M, Khazenzon NM, Lee BS, WachsmannHogiu S, Farkas DL, Black KL, Hollerb E. Nanoconjugate based on polymalic acid for tumor targeting. Chem Biol Interact. 2008; 171(2):195–203
