Approaches to gene identification of Campylobacteria genus bacteria by polymerase chain reaction with universal primers

Authors

  • D. L. Kirik Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine 5, Amosova Str., Kyiv, Ukraine, 03038 Author
  • L. N. Burianovsky Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • E. A. Sliablovskaya Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine 5, Amosova Str., Kyiv, Ukraine, 03038 Author
  • I. V. Pinchuc Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine 5, Amosova Str., Kyiv, Ukraine, 03038 Author
  • N. M. Krolevetskaya Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine 5, Amosova Str., Kyiv, Ukraine, 03038 Author
  • V. I. Kikot Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine 5, Amosova Str., Kyiv, Ukraine, 03038 Author

DOI:

https://doi.org/10.7124/bc.000424

Abstract

Preliminary studies on development of gene identification method of Campylobacteria genus bacteria on the base of polymerase chain reaction (PCR) with using universialoligonucleotide primers – REP (repetitive extragenic palindromic sequences). For all strains of Campylobacter jejuni PCR-product was peculiar 600 pairs ofnucleotides. Five group ofPCR-serovars in strains C. jejuni, one serovar (Lio 32) differed on minoric fragments. It can be of importance for difinition of infection source while epidemic analysis.

References

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Published

1996-03-20

Issue

Vol. 12 No. 2 (1996)

Section

Genome and Its Regulation