Mapping of Bacillus subtilis riboflavin operon fragment coding for 3,4-dihydroxy-2-butanone-4-phosphate synthase
DOI:
https://doi.org/10.7124/bc.000414Abstract
Complementation analysis of Bacillus subtilis riboflavin operon was carried out by means of Escherichia coli riboflavin deficient strains and recombinant plasmids. It was found that ORF-3 ofB. subtilis riboflavin operon encodes for a bifunctional enzyme. It catalyses formation of both 2, 5-diamino-4-hydroxy-6-ribosylamino-pyrimidine-5'-phosphate from GTP and 3, 4-dihydroxy-2-butanone-4-phosphate from ribuhse-5-phosphate.References
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