Study on occurrence of the IVS8-5T allele of the CFTR gene in Ukrainian males with spermatogenesis failure

Authors

  • O. A. Fesai Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • S. A. Kravchenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • V. M. Zinchenko "ISIDA" clinic 65, Ivana Lepse Boulevard, Kyiv, Ukraine, 03126 Author
  • L. A. Livshits Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000164

Keywords:

male infertility, spermatogenesis failure, IVS8-nT polymorphism

Abstract

Aim. To study the IVS8-5T allele of the CFTR gene and it is involvement in spermatogenesis failure in men with azoospermia and oligozoospermia. Methods. The IVS8-nT polymorphism was analyzed by PCR followed by «A.L.F.-express» fragment analysis in the infertile men group, consisting of 113 azoospermic and 217 oligozoospermic patients, and the control group of 150 fertile men with proven paternity. Results. The frequency of the IVS8-5T allele among infertile males was higher than in controls. A statistically significant difference (P < 0.05) was observed in the frequencies of the IVS8-5T allele in azoospermia patients (5.3 %) when compared with the control group (2.0 %). Conclusions. The IVS8-5T allele of the CFTR gene contributes to spermatogenesis failure and/or sperm maturation.
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Published

2010-07-20

Issue

Vol. 26 No. 4 (2010)

Section

Molecular Biomedicine