Artificial skin equivalent based on copolymeric hydrogel membranes with immobilized human mesenchymal stem cells

Authors

  • O. O. Kosenko Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • L. L. Lukash Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • Yu. M. Samchenko Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • T. A. Ruban Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • S. I. Lukash Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • Z. R. Ulberg Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • N. P. Galagan Institute of Surface Chemistry, NAS of Ukraine 17, Generala Naumova Str., Kyiv, Ukraine, 03164 Author

DOI:

https://doi.org/10.7124/bc.00074B

Keywords:

skin equivalent, hydrogel, mesenchymal stem cells, immobilization, cultivation, nutrient medium

Abstract

The basic parameters of hydrogel membranes, which are important for their characteristics as potential biocompatible non-degradable wound coverings, have been optimized. Moderately hydrophobic and at the same time moderately hydrophilic membranes obtained on the basis of copolymerization of acrylamide and acrylonitrile were demonstrated to be biocompatible with mesenchymal stem cells. Small concentrations of highly dispersed silica introduced into a composition of hydrogel membranes were demonstrated to influence the cell cultivation positively. The technological conditions necessary for more effective and prolonged cultivation of cells on the surface of hydrogel membranes with improved exploitation parameters have been established

References

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Published

2006-11-20

Issue

Vol. 22 No. 6 (2006)

Section

Molecular and Cell Biotechnologies