Search for the modification-restriction systems in Agrobacteria DNA

Authors

  • T. V. Stefanishina Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • I. G. Bogdarina Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR Pushchino, Moscow Region, USSR Author
  • Ya. I. Buriyanov Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR Pushchino, Moscow Region, USSR Author

DOI:

https://doi.org/10.7124/bc.0001DF

Abstract

The DNA modification-restriction systems have been studied for their presence in 19 Agrobacieria strains, including the virulent ones with 77-plasmids of various classes, the avirulent octopine and nopaline strain derivatives obtained by curing the Agrobacteria virulent strains from 77-plasmids as well as natural isolates of avirulent Agrobacteria. It is shown that the presence of the DNA modification-restriction system with EcoRIl specificity is a characteristic feature of the Agrobacteria octopine strains and of their cured avirulent derivatives. The Agrobacteria nopaline strains, their cured avirulent derivatives, and most of the Agrobacteria natural avirulent strains contain the DNA modification-restriction systems different from EcoRIl. The chromosomal nature of these characters is shown.

References

Vervliet G, Holsters M, Teuchy H, Van Montagu M, Schell J. Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J Gen Virol. 1975;26(1):33-48.

Casse F, Boucher C, Julliot JS, Michel M., Denarie J. Identification and Characterization of Large Plasmids in Rhizobium meliloti using Agarose Gel Electrophoresis. J Gen Microbiol. 1979; 113(2):229-242

Schleif R. Assaying of organisms for the presence of restriction endonucleases. Methods Enzymol. 1980;65(1):19-23.

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1985;13 Suppl:r165-200.

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Published

1987-05-20

Issue

Vol. 3 No. 3 (1987)

Section

Short Communications