Development of enzyme mono- and multi-biosensor based on a light addressable potentiometric sensor

Authors

  • S. V. Patskovsky Taras Shevchenko National University of Kyiv 64, Volodymyrska Str., Kyiv, Ukraine, 01033 Author
  • G. A. Zhylyak Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • V. V. Volotovsky Taras Shevchenko National University of Kyiv 64, Volodymyrska Str., Kyiv, Ukraine, 01033 Author
  • V. I. Strikha Taras Shevchenko National University of Kyiv 64, Volodymyrska Str., Kyiv, Ukraine, 01033 Author

DOI:

https://doi.org/10.7124/bc.000562

Abstract

In Ms work a light addressable potentiometric sensor (LAPS) has been considered as a basis for creation of biosensors. The opportunity of development of the mono- and tnultienzyme sensors with the help of LAPS has been shown. The results received confirm prospect of this direction in comparison with use of traditional ion-sensitive field effect transistors.

References

Hafeman DG, Parce JW, McConnell HM. Light-addressable potentiometric sensor for biochemical systems. Science. 1988;240(4856):1182-5.

Bousse L, Owicki JC, Parce JW. Biosensors with microvolume reaction chambers. Chemical Sensor Technology. 1992;145–66.

Dzyadevich SV, Shul'ga AA, Patskovsky SV, Arkhipova VN, Soldatkin AP, Strikha VI. Thin-film conductometric transducer for enzyme biosensors. Elektrokhimiia. 1994. 30(8):982—7.

Patskovskiy SV, Strika VI. Multibiosensor with light-addressable. Rep. scientific and practical. Conf. "High technology dual-use" (19-21 Apr, 1994). Kiev: KVIUZ, 1994; 164 p.

Adami M, Sartore M, Nicolini C. PAB: a newly designed potentiometric alternating biosensor system. Biosensors and Bioelectronics. 1995;10(1-2):155–67.

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Published

2000-03-20

Issue

Vol. 16 No. 2 (2000)

Section

Methods