High selectivity of mutagenic action of synthetic polynucleotides
DOI:
https://doi.org/10.7124/bc.000522Abstract
Solutions of high molecular weight syntetic poiyribo- and polydeoxyribonucleotides of known chemical structure injected into Drosophila melanogaster males caused a 6–17 fold increase of the overall frequency of the recessive lethals in the 2nd chromosome. Complementation analysis of the induced lethals showed an extremely high locus-specificity for some of these mutagens. Possible mechanisms of the mutagenic action of synthetic polynucleotides are discussed.References
Gershenson SM, Alexandrov YuN, Malyuta SS. Mutagenic Effect of DNA and viruses in Drosophila. Kiev: Naukova Dumka, 1975; 160 p.
Gershenson, S.M. Mutagenic action of DNA, insertion, transposition and gene instability (1980) Proc. 14 Int. Congr. of Genet, 1, Moscow: Mir, book 2 pp. 91-115.
Fahmy OG., Fahmy MJ. Mutagenic activity of cellular macromolecules in drosophila melanogaster (1962) Nature, 196 (4857), pp. 873-876.
Fahmy, O.G., Fahmy, M.J. Mutagenesis in relation to nucleic acid synthesis in cellular genetic system (1966) Mechanisms of mutation and inducing factors, Prague: Academia pp. 267-282.
Simmons MJ, Crow JF. Mutations affecting fitness in Drosophila populations. Annu Rev Genet. 1977;11:49-78.
Abrahamson S, Würgler FE, DeJongh C, Meyer HU. How many loci on the X-chromosome of Drosophila melanogaster can mutate to recessive lethals? Environ Mutagen. 1980;2(4):447-53.
Ives PT. The Genetic Structure of American Populations of Drosophila Melanogaster. Genetics. 1945;30(2):167-96.
Wallace B. Allelism of second chromosome lethals in D. melanogaster. Proc Natl Acad Sci U S A. 1950;36(11):654-7.
Cohen LH, Penner PE, Loeb LA. Multiple DNA polymerases displayed by isoelectric focusing. Ann N Y Acad Sci. 1973;209:354-62.
Margulies L, Chargaff E. Survey of DNA polymerase activity during the early development of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1973;70(10):2946-50.
Gershenson SM, Alexandrov YuN. Mutagenic effect of natural and synthetic polynucleotides and the problem of directed mutations (1982) ZH. OBSHCH. BIOL., 43 (6), pp. 747-763.
Alexandrov YN. Natural and synthetic polynucleotides of selective action (1988) Mutagenic action of natural and synthetic polynucleotides, Ed. V. V. Morgun.-Kiev: Nauk. Dumka, pp. 4-10.
Gerasimova TI, Ilyin YV, Misrokhi LI, Semenova LV, Georgie GP. A direct demonstration of transposition of a mobile element and its role in the appearance of unstable mutations in Drosophila. Dokl. Akad. Nauk SSSR., 1983; 271, pp. 977-980.
Gerasimova TI, Mizrokhin LIu, Georgiev GP. «Transposition bursts» in single germ cells in genetically destabilized strains of Drosophila melanogaster. Dokl Akad Nauk SSSR, 1984; 274(6):1473-6.
Gerasimova T.I. Transposition of mobile elements and their role in insertion mutagenesis in Drosophila (1985) Stability and variability of the genome, Moscow: Nauka, pp. 26-39.
Gerasimova TI, Mizrokhi LY, Obolenkova LA, Georgiev GP. Directed mutagenesis under the influence of mobile elements in unstable strains of Drosophila melanogaster. Dokl. Akad. Nauk SSSR., 1985; 280, pp. 1246-1250.
Gerasimova TI, Mitin VA, Churikov NA, Kochieva EZ, Georgiev GP. Site-directed mutagenesis in the cut locus of Drosophila melanogaster controlled by the mobile element mdg4. Dokl Akad Nauk SSSR. 1988;300(2):462-5.
Gerasimova TI, Ladvishchenko AB, Mogila VA, Georgieva SG, Kiselev SL, Maksymiv DV. Transpositional bursts and chromosome rearrangements in unstable lines of Drosophila Genetika. 1990;26(3):399-411.
Green, M.M. Mobile elements and spontaneous gene mutations (1988) Bambury Rep. 30: Eukaryotic transposable elements as mutagenic agents,New York: Cold Spring Harbor Lab pp. 41-50.
