Immunochemical identification of apoprotein A-I in the chromatin of rat hepatocyte nuclei

Authors

  • L. E. Panin Institute of Biochemistry Russian Academy of Medical Sciences Str. Timakov 2, Novosibirsk, Russian Federation, 630117 Author
  • L. M. Polyakov Institute of Biochemistry Russian Academy of Medical Sciences Str. Timakov 2, Novosibirsk, Russian Federation, 630117 Author
  • I. Ph. Usynin Institute of Biochemistry Russian Academy of Medical Sciences Str. Timakov 2, Novosibirsk, Russian Federation, 630117 Author
  • A. P. Kuzmenko Institute of Biochemistry Russian Academy of Medical Sciences Str. Timakov 2, Novosibirsk, Russian Federation, 630117 Author
  • O. N. Poteryaeva Institute of Biochemistry Russian Academy of Medical Sciences Str. Timakov 2, Novosibirsk, Russian Federation, 630117 Author

DOI:

https://doi.org/10.7124/bc.000319

Abstract

Two proteins of 28000 and 14000 immunologically identifical to apoprotein A-I were found in the chromatin of rat hepatocyte nuclei of using immunoblotting and enzyme-linked immunosorbent assay. The 28 000 protein was present in the trans-criptionally active chromatin and in the nuclear matrix. The 14000 protein was present in the repressed chromatin. The molecular weights of these protein determinated by SDS-PAGE, and immunochemical identity allow us to propose, that one of this proteins is apoprotein A-I, and the other one is probably a product of limited proteolysis of apoprotein A-I.

References

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Published

1992-03-20

Issue

Vol. 8 No. 2 (1992)

Section

Cell Biology