The effect of modification of terminal groups of oligonucleotides on their stability in mycoplasma culture
DOI:
https://doi.org/10.7124/bc.0002F0Abstract
The effect of modification of terminal groups of deoxyribooligonucleotides on their stability in culture medium and mycoplasma cells A. laldlawii PQ-8 and M. capricolum California kid has been investigated. The oligonucleotides and their derivatives were stable in culture medium with 10 % horse serum during 24 hours. In the medium with mycoplasms, orthophosphates were rapidly removed from the 5'-ends and the oligonucleotides degraded to mononucleotides. In the cells, the scission of 5'-phosphomono-ester bonds was accompanied by reutilization of the phosphate. Attachment of the cholesterol or phenazinium residues to oligonucleotides 3'- or 5'-ends protected them from nucleases. The modification of oligonucleotides 5'-ends by 5'-phosphoramides formation, protect the oligonucleotides from dephosphorylation and slower the degradation down. Oligonucleotides protected from 3'- or 5'-ends are more stable to mycoplasma nucleases.References
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