Isolation and characterization of extracellular vesicles from different types of mesenchymal stromal/stem cells for future translation research

Authors

  • I. M. Gordiienko R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine 45, Vasilkivska Str., Kyiv, Ukraine, 01022; Biotechnology Laboratory, Medical Company «Good Cells» 9, Ivana Kramskoho Str., Kyiv, Ukraine, 03115 Author
  • M. V. Shamshur Biotechnology Laboratory, Medical Company «Good Cells» 9, Ivana Kramskoho Str., Kyiv, Ukraine, 03115; Institute of Genetic and Regenerative Medicine, NAMS of Ukraine 67, Vyshhorodska Str., Kyiv, Ukraine, 04114; M. D. Strazhesko National Scientific Center of Cardiology, Clinical and Regenerative Medicine, NAMS of Ukraine 5, Sviatoslava Khorobroho Str., Kyiv, Ukraine, 03151 Author
  • N. M. Petryk Biotechnology Laboratory, Medical Company «Good Cells» 9, Ivana Kramskoho Str., Kyiv, Ukraine, 03115 Author
  • A. V. Zlatska Biotechnology Laboratory, Medical Company «Good Cells» 9, Ivana Kramskoho Str., Kyiv, Ukraine, 03115; Institute of Genetic and Regenerative Medicine, NAMS of Ukraine 67, Vyshhorodska Str., Kyiv, Ukraine, 04114; M. D. Strazhesko National Scientific Center of Cardiology, Clinical and Regenerative Medicine, NAMS of Ukraine 5, Sviatoslava Khorobroho Str., Kyiv, Ukraine, 03151 Author

DOI:

https://doi.org/10.7124/bc.000ACD

Keywords:

extracellular vesicles, mesenchymal stromal/stem cells, exosomes, growth factors, cytokines

Abstract

Aim. The aim of our study was to isolate and characterize EVs from umbilical cord-derived MSCs (UC-MSCs), adipose tissue-derived MSCs (ADSCs) and dental pulp stem cells (DPSCs). Conclusions. UC-MSCs and DPSCs are the most suitable cell types for large-scale EVs isolation. Based on size distribution and CD63/CD81 expression, EVs isolated from UC-MSCs, DPSCs, and ADSCs can be defined as exosomes. UC-MSC-derived EVs contain higher levels of growth factors and cytokines compared to DPSC- and ADSC-derived EVs, which may mediate stronger tissue regeneration through regulation of cell proliferation, immunosuppression, and vasculogenesis.
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Published

2024-09-10

Issue

Vol. 40 No. 3 (2024)

Section

Chronicle and Information