Production of recombinant proteins in plant systems through transient gene expression technology

Authors

  • Ya. R. Sindarovska Institute of Cell Biology and Genetic Engineering, NAS of Ukraine 148, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 Author
  • M. V. Kuchuk Institute of Cell Biology and Genetic Engineering, NAS of Ukraine 148, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 Author

DOI:

https://doi.org/10.7124/bc.000AE0

Keywords:

recombinant proteins, transient gene expression, plant expression systems, <em>Nicotiana benthamiana</em>, biopharming

Abstract

Aim. The transient gene expression (TGE) is a widely used method for plant biopharming that allows the production of high levels of recombinant proteins within days. The aim of the work was to investigate the influence of factors on TGE and to develop an effective protocol for the production of recombinant proteins in plant systems. Conclusions. The production of recombinant proteins, including valuable ones, in plant systems can reach high levels after optimization of several parameters of biotic and abiotic nature. We selected the factors with the strongest effect on TGE and, after optimizing them, increased the levels of the target recombinant proteins.

References

Sheludko YV, Sindarovska YR, Gerasymenko IM, Bannikova MA, Kuchuk NV. Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression. Biotechnol Bioeng. 2007; 96(3):608-14.

Sindarovska Y, Kuchuk M. Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro. Plants (Basel). 2021; 10(10):2187.

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Published

2024-09-10

Issue

Vol. 40 No. 3 (2024)

Section

Chronicle and Information