Interaction between short oligonucleotides and recombinant signaling proteins and their receptors

Authors

  • O. O. Nuzhnyi Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 Author
  • R. O. Nikolaiev Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 Author
  • Z. Yu. Tkachuk Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 Author

DOI:

https://doi.org/10.7124/bc.000ABA

Keywords:

interferon, receptor, insulin, somatotropin, protein–ligand interaction, spectroscopy, oligonucleotide, docking

Abstract

Aim. Oligonucleotides are short fragments of DNA or RNA that play a significant role in many fundamental biological processes and have pharmacological activity [1]. The aim of this study was to investigate the interaction of synthetic purine ribo- and deoxyoligonucleotides (OLNs) with recombinant signaling proteins, in particular interferon α-2b and insulin, their receptors, and somatropin. Conclusions. The results of our experiments indicate that OLNs interact with recombinant signaling proteins and their receptors by different mechanisms (single-, double-ligand positive and negative cooperative) depending on the protein and the ligand itself, which, in turn, may be important for their various therapeutic applications.

References

Tkachuk Z. Multiantivirus Compound, Composition and Method for Treatment of Virus Diseases. U.S. Patent 20,120,232,129. 2013.

Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd ed. Springer; 2006.

Murcko, MA. Computational Methods to Predict Binding Free Energy in Ligand-Receptor Complexes. J Med Chem. 1995; 38(26):4953-67.

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Published

2024-09-10

Issue

Vol. 40 No. 3 (2024)

Section

Chronicle and Information