The efficient method for DNA encapsulation into reconstituted sendai virus envelopes

Authors

  • V. V. Vlassov Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk, USSR Author
  • Yu. D. Krendelev Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk, USSR Author
  • M. N. Ovander Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • A. S. Ryte Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk, USSR Author
  • V. E. Repin Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk, USSR Author
  • F. P. Svinarchuk Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR Novosibirsk, USSR Author

DOI:

https://doi.org/10.7124/bc.000233

Abstract

The freezing-thawing method is used for encapsulation up to 17000 base pairs DNA into reconstituted Sendai virus envelopes (RSVE). It permits encapsulating up to 30 % of DNA added to the solution. The interaction RSVE loaded with plasmid DNA and Krebs-2 ascites tumours cells results in up to 30 % penetration of the encapsulated material into the cells.

References

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Published

1988-09-20

Issue

Section

Structure and Function of Biopolymers