Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol
DOI:
https://doi.org/10.7124/bc.000128Keywords:
tRNA<sub>3</sub><sup>Lys</sup>, lysyl-tRNA synthetase, HIV-1, packagingAbstract
Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA3Lys:GagPol packaging complex is proposed.References
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