Comet assay application for determining genotoxicity of veterinary vaccines preparations

Authors

  • S. M. Dybkova Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • M. E. Roman'ko Institute of Experimental and Clinical Veterinary Medicine, UAAS 83, Pushkinska Str., Kharkov, Ukraine, 61023 Author
  • T. G. Gruzina Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • L. S. Rieznichenko Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • Z. R. Ulberg Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine 42, Vernadsky blvd., Kyiv, Ukraine, 03142 Author
  • V. O. Ushkalov Institute of Experimental and Clinical Veterinary Medicine, UAAS 83, Pushkinska Str., Kharkov, Ukraine, 61023 Author
  • A. M. Golovko The state scientific control institute of biotechnology and strains of microorganisms 30, Donets'ka Str., Kyiv, Ukraine, 03151 Author

DOI:

https://doi.org/10.7124/bc.000157

Keywords:

genotoxicity, veterinary vaccines, cells culture, method of alkaline single cells gel electrophoresis, biosafety

Abstract

Aim. To study a possibility of usage of the alkaline gel electrophoresis method (Comet assay) for determination of genotoxic properties of veterinary vaccines preparations. Methods. The alkaline gel electrophoresis of isolated eukaryotic cells has been used with further visualization of the samples by fluorescent microscopy. Results. Veterinary vaccines testing by the Comet assay method under alkaline conditions revealed that the samples No.1 and No 12 from twelve investigated vaccine preparations had genotoxic influence on eukaryotic cells of CHO-K1 and Vero test cultures, No. 12 sample being genotoxic only at metabolic activation. Conclusions. The method of alkaline gel electrophoresis of single cells (Comet assay) is suitable for determination of veterinary vaccines genotoxic influence on the test eukaryotic cells CHO-K1. The method proposed in this paper is express, inexpensive and predictive. The accompli-hed experimental work allows us to recommend this method for characterization of biosafety of veterinary vaccines preparations.

References

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Published

2010-05-20

Issue

Vol. 26 No. 3 (2010)

Section

Molecular and Cell Biotechnologies