Application of carbocyanine fluorescent probes for evaluation of the functional state of cell cultures after cryoconservation

Authors

  • E. I. Goncharuk Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • E. V. Onischenko Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • V. V. Timon Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • I. A. Borovoy State Scientific Institution "Institute for Single Crystals" NAS of Ukraine 60, Lenin Ave., Kharkiv, Ukraine, 61001 Author
  • Yu. V. Maliukin State Scientific Institution "Institute for Single Crystals" NAS of Ukraine 60, Lenin Ave., Kharkiv, Ukraine, 61001 Author
  • V. I. Grishchenko Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author

DOI:

https://doi.org/10.7124/bc.0007A4

Keywords:

fluorescence, probes, human fibroblasts, cell culture, cryoconservation

Abstract

Application of C2, C9 and JC-1 dyes for characterizing human fibroblast diploid cell line after cryoconservation was studied. It was shown that these probes might be used to monitor the functional state of cryocenserved cells.

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Published

2008-05-20

Issue

Section

Cell Biology