Effects of interferon-α/β on Ca2+ in flux and binding in murine thymocytes

Authors

  • E. V. Dolgaya Bogomoletz Institute of Physiology, NAS of Ukraine 4, Bogomolets Str., Kyiv, Ukraine, 01024 Author
  • O. M. Rozhmanova Bogomoletz Institute of Physiology, NAS of Ukraine 4, Bogomolets Str., Kyiv, Ukraine, 01024 Author
  • L. N. Stelmakh Bogomoletz Institute of Physiology, NAS of Ukraine 4, Bogomolets Str., Kyiv, Ukraine, 01024 Author
  • A. V. Miransky Bogomoletz Institute of Physiology, NAS of Ukraine 4, Bogomolets Str., Kyiv, Ukraine, 01024 Author
  • Yu. J. Kudryavets R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine 45, Vasilkivska Str., Kyiv, Ukraine, 01022 Author

DOI:

https://doi.org/10.7124/bc.00056A

Abstract

In order to determine the role of calcium in interferon (IFN)-induced immunomodulation, we investigated calcium transport and binding induced by murine IFN-α/β in murine thymocytes. By radiometric method, it was found that a rapid, more than 5-fold, increase in 45Ca2+ influx developed and reached a plateau within 10 min. At the same time the amount of 45Ca2+ associated with the cell surface half decreased. During next 30 min the recovery of 45Ca2+ binding to control level in INF-induced lymphocytes occured. 45Ca2+ influx in thymocytes was dose-dependent. The maximal increase in calcium influx was observed at the IFN concentration of 600 W/ml. Pretreatment of thymocytes with a calcium channel blacker, verapamil, at the doses about 30mkM for 20 min before IFN application significantly decreased 45Ca2+ influx. Depolarization of thymocytes up to 25 mM by increasing the extracellular K+ concentration resulted in complete inhibition of 45Ca2+ influx. Our data indicate that in murine thymocytes IFN-induced calcium influx occcured via voltage-operated calcium channels.

References

Smorodintsev AA, Iovlev VI, Stepanov AN. Interferon. Itogi Nauki i Tekhniki (Virusologiia; Vol. 13). M.: VINITI, 1987 .

Ashman RF. Lymphocyte activation, in Fundamental Immunology, Paul WE Ed Raven Pres New York 1984.

De Maeyer-Guignard J, De Maeyer E. Immunomodulation by interferons: recent developments. Interferon. 1985;6:69-91.

Singh VK, Maheshwari RK, Krisima G, Friedman RM. Effects of interferon on calcium. The 2-5 A molecular and clinical aspects of the interferon-regulated pathway. New York: Alan Liss. Inc., 1985: 351-6.

Martino G, Brambilla E, Filippi M, Martinelli V, Colombo B, Rodegher M, Comi G, Grimaldi LM. Interferon-gamma activated calcium influx in peripheral blood lymphocytes from patients with primary and secondary progressive multiple sclerosis. J Neurol Neurosurg Psychiatry. 1996;61(5):515-7.

Aas V, Larsen K, Iversen JG. IFN-gamma induces calcium transients and increases the capacitative calcium entry in human neutrophils. J Interferon Cytokine Res. 1998;18(3):197-205.

Knight E Jr, Korant BD. A cell surface alteration in mouse L cells induced by interferon. Biochem Biophys Res Commun. 1977;74(2):707-13.

B?yum A. Separation of leukocytes from blood and bone marrow. Introduction. Scand J Clin Lab Invest Suppl. 1968;97:7.

Iwagaki H, Fuchimoto S, Miyake M, Aoki H, Orita K. Interferon-gamma activates the voltage-gated calcium channel in RPMI 4788 cells. Biochem Biophys Res Commun. 1988;153(3):1276-81.

Klein JB, McLeish KR, Sonnenfeld G, Dean WL. Potential mechanisms of cytosolic calcium modulation in interferon-gamma treated U937 cells. Biochem Biophys Res Commun. 1987;145(3):1295-30.

Hansen AB, Bouchelouche PN, Lillevang ST, Andersen CB. Interferon-gamma increases cellular calcium ion concentration and inositol 1,4,5-trisphosphate formation in human renal carcinoma cells: relation to ICAM-1 antigen expression. Br J Cancer. 1994;69(2):291-8.

Kostyuk PG. Calcium and cellular excitability. M.: Nauka, 1986;5-26.

Russell AJ, Fersht AR. Rational modification of enzyme catalysis by engineering surface charge. Nature. 1987 Aug 6-12;328(6130):496-500.

biopolym.cell-2000-16-3-cover.png

Downloads

Published

2000-05-20

Issue

Vol. 16 No. 3 (2000)

Section

Structure and Function of Biopolymers