Cloning and bacterial expression of the cytokine-like noncatalytic domain of bovine tyrosyl-tRNA synthetase
DOI:
https://doi.org/10.7124/bc.0004EAAbstract
Cytokine-like noncatalytical C-terminal domain of mammalian tyrosyl-tRNA synthetase (D322-S528) has been cloned and expressed in bacterial expression system using pET15b vector. Recombinant protein has been isolated by metal-chelating chromatography on the Ni-NTA agarose into a homogenous state according to gel-electrophoresis data.References
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