Biopolym. Cell. 2008; 24(5):385-392.
http://dx.doi.org/10.7124/bc.0007B6
Structure and Function of Biopolymers
Study on interaction of Thermus thermophilus prolyl-tRNA synthetase with gomologous tRNACGGPro by methods of chemical modification in solution
1Egorova S. P., 1Krikliviy I. A., 1Kovalenko O. P., 1Yaremchuk A. D., 1Tukalo M. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Two isoaccepting Thermus thermophilus HB8 tRNAPro were isolated by the chromatography methods with purity about 95 and 97 % . The primary structures of isoaccepting tRNAGGGPro and tRNACGGPro were studied by the gel-sequencing method, and differences between them were found in 18 positions. Our results show that in solution the gomologous prolyl-tRNA synthetase protects the phosphates allocated in D-stem (9, 10, 13), 5'-end of anticodon-stem (26–29), anticodon-loop (34, 35, 37–39) and acceptor-stem (67, 68) of tRNACGGPro from alkylation by ethylnitrosourea.
Keywords: prolyl-tRNA synthetase, tRNAPro, Thermus thermophilus, chromatography, autoradiography, ethylnitrosourea
Full text: (PDF, in English) (PDF, in Ukrainian)

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