Phagedependent overproduction of β-galactosidase Escherichia coli and working out its purification

Chernykh, S. I.; Slavchenko, I. Yu.; Gorlov, Yu. I.; Terent'ev, A. G.; Gavrish, T. G.; Kordyum, V. A.

doi:10.7124/bc.0004C4

Biopolym. Cell. 1998; 14(2):127-131.

http://dx.doi.org/10.7124/bc.0004C4

Structure and Function of Biopolymers

Phagedependent overproduction of β-galactosidase Escherichia coli and working out its purification

1Chernykh S. I., 1Slavchenko I. Yu., 1Gorlov Yu. I., 1Terent'ev A. G., 1Gavrish T. G., 1Kordyum V. A.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The phagedependent method of obtaining of β-galactosidase (EC 3.2.1.23) E. coli has been worked out. The yield was up 700 U of enzyme activity per ml of cultural fluid. Accumulation of enzyme in cultural medium has permitted to use membrane method for preliminary purification of β-galactosidase. Concentrated and partially purified preparation of β-galactosidase has been purified by ligand-affinity chromatography. Pure preparation of β-galactosidase has characterized by high purity and nativeness. The process of obtaining and purification of β-galactosidase were made in pilot scale.

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