Biopolym. Cell. 1988; 4(1):44-48.
Gene-Engineering Biotechnology
Homology between structural nitrogenase genes (nifHDK) from Klebsiella pneumoniae and extrachromosomal dnas from microsymbiont vesicles of Alnus glutinosa
1Dobritsa S. V., 1Tomashevskii A. Yu.
  1. Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

The fraction of circular extrachromosomal DNAs of 14 classes detected previously in vesicles of a Frankia microsymbiont from nitrogen-fixing root nodules of black alder was hybridized with [32P] DNA of the plasmid pSASO carrying K. pneumoniae nifHDK genes. Circular DNAs of two classes (about 6 and 12 kb) were shown to contain sequences homologous to the nitrogenase genes. Possible genome rearrangements inducing multiple circular DNAs in vesicles are discussed.

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