Biopolym. Cell. 1988; 4(1):44-48.
Gene-Engineering Biotechnology
Homology between structural nitrogenase genes (nifHDK) from Klebsiella pneumoniae and extrachromosomal dnas from microsymbiont vesicles of Alnus glutinosa
1Dobritsa S. V., 1Tomashevskii A. Yu.
  1. Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR


The fraction of circular extrachromosomal DNAs of 14 classes detected previously in vesicles of a Frankia microsymbiont from nitrogen-fixing root nodules of black alder was hybridized with [32P] DNA of the plasmid pSASO carrying K. pneumoniae nifHDK genes. Circular DNAs of two classes (about 6 and 12 kb) were shown to contain sequences homologous to the nitrogenase genes. Possible genome rearrangements inducing multiple circular DNAs in vesicles are discussed.


[1] Becking H. Nitrogen fixation in nodulated plants other than legumes. Adv. in agricult. microbiol. Ed. N. S. Subba Rao. London: Butterworth Scientific, 1982:89-110.
[2] Torrey JG, Callaham D. Structural features of the vesicle of Frankia sp. CpI1 in culture. Can J Microbiol. 1982. 28(7):749-57.
[3] Meesiers TM. Localization of nitrogenase in vesicles of Frankia sp. Cc1.17 by immunogoldlabelling on ultrathin cryosections. Arch. Microbiol. 1987. 146(4):327-31.
[4] Dobritsa SV. Extrachromosomal circular DNAs in endosymbiont vesicles from Alnus glutinosa root nodules. FEMS Microbiol Lett. 1982; 15(2):87-91.
[5] Dobritsa SV. Molecular genome organization Frankia. Biochemistry and Physiology of Microorganisms. Ed. GK Skriabina. Pushchino, 1985; 294-302.
[6] Ruvkun GB, Ausubel FM. Interspecies homology of nitrogenase genes. Proc Natl Acad Sci U S A. 1980;77(1):191-5.
[7] Vaisanen O, Haahtela K, Bask L, Kari K, Salkinoja-Salonen M, Sundman V. Diversity of nif gene location and nitrogen fixation among root-associated Enterobacter and Klebsiella strains. Arch Microbiol. 1985; 141(2):123-7.
[8] Tomashevskii AIu, Dobritsa SV. Hybridization of DNA from Actinomycetes of the genus Frankia with nitrogenase structural genes (nifHDK) of Klebsiella pneumoniae and nod-genes of Rhizobium melioti. Mol Gen Mikrobiol Virusol. 1987;(3):27-32.
[9] Cannon FC, Riedel GE, Ausubel FM. Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized. Mol Gen Genet. 1979;174(1):59-66.
[10] Dobritsa S. V. Restriction analysis of the Frankia spp. genome. FEMS Microbiol Lett. 1985; 29(1-2):123-8.
[11] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.
[12] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.
[13] Nuti MP, Lepidi AA, Prakash PK, Schilperoort RA, Cannon FC. Evidence for nitrogen fixation (nif) genes on indigenous Rhizobium plasmids. Nature. 1979; 282(5738):533-5.
[14] Normand P, Simonet P, Butour JL, Rosenberg C, Moiroud A, Lalonde M. Plasmids in Frankia sp. J Bacteriol. 1983;155(1):32-5.
[15] Simonet P, Normand P, Moiroud A, Lalonde M. Restriction enzyme digestion patterns of Frankia plasmids. Plant and Soil. 1985; 87(1):49-60.
[16] Normand P, Lalonde M. The genetics of actinorhizal Frankia: A review. Nitrogen fixation with non-legumes. Eds F. A. Skinner, P. Uomala. Dordrecht: Martinus Nijhoff Publ., 1986:429-53.
[17] Simonet P, Haurat J, Normand P, Bardin R, Moiroud A. Localization of nif genes on a large plasmid in Frankia sp. strain ULQ0132105009. Mol Gen Genet. 1986; 204(3):492-5.
[18] Starodubtseva LI, Danilenko VN, Navashin SM. Plasmid and genomic instability Streptomyces. Antibiotiki. 1984; 29(7):536-55.
[19] Koukolikova-Nicola Z, Shillito RD, Hohn B, Wang K, Van Montagu M, Zembryski P. Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells. Nature. 1985; 313(5999):191-66.
[20] Golden JW, Robinson SJ, Haselkorn R. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature. 1985 Apr 4-10;314(6010):419-23.
[21] Ligon JM, Nakas JP. Isolation and characterization of the genes from Frankia that code for the component I proteins of nitrogenase. Nitrogen fixation research progress. Eds H. J. Evans, P. J. Bottomley, W. E. Newton. Dordrecht: Martinus Nijhoff Publ., 1985:188.
[22] Quinto C, de la Vega H, Flores M, Fernandez L, Ballado T, Soberon G, Palacios R. Reiteration of nitrogen fixation gene sequences in Rhizobium phaseoli. Nature. 1982; 299(5885):724-6.
[23] Scolnik PA, Haselkorn R. Activation of extra copies of genes coding for nitrogenase in Rhodopseudomonas capsulata. Nature. 1984 Jan 19-25;307(5948):289-92.