Biopolym. Cell. 1987; 3(3):137-139.
http://dx.doi.org/10.7124/bc.0001E2
Short Communications
Concanavalin-A application for the isolation of glycoproteins and their electrophoretic separation parallel with other proteins
1Golovatsky I. D., 1Savchik B. A.
  1. A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

A combination of the methods for precipitation of glycoproteins by concanavalin-A. for their release by monosaccharides (glucose, mannose) and for subsequent separation in the polyacrylamide gel has been used for simultaneous and parallel determination of certain glycoproteins and other .tissue proteins. A good reproducibility of the results is established. 10–20 fractions of glycoproteins being, mainly, in bands corresponding to other proteins are shown to be present in different rat and bovine tissues.
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