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Concanavalin-A application for the isolation of glycoproteins and their electrophoretic separation parallel with other proteins
- A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
A combination of the methods for precipitation of glycoproteins by concanavalin-A. for their release by monosaccharides (glucose, mannose) and for subsequent separation in the polyacrylamide gel has been used for simultaneous and parallel determination of certain glycoproteins and other .tissue proteins. A good reproducibility of the results is established. 10–20 fractions of glycoproteins being, mainly, in bands corresponding to other proteins are shown to be present in different rat and bovine tissues.
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