Biopolym. Cell. 1993; 9(6):71-74.
Cell Biology
The influence of photosensitizing action on lymphocytes receptor properties
1Shevchenko A. A., 1Lobanok E. S., 1Vorobey A. V.
  1. Institute of Photobiology, Academy of Sciences of Belarus
    27, Academicheskaya, Minsk, Republic of Belarus, 220072


Effect of illumination in the presence of the sensitizers (hematopor-phyrin and merocyanine 540) on phytohemagglutinin-induced lymphocytes agglutination and lectin receptors capping was studied. The increase of velocity of both processes under non-lethal of illumination has been shown. A possible role of cytoskeletal proteins damage in the photomodification of cell receptor characteristics is discussed.


[1] Dougherty TJ. Photodynamic therapy. Clin Chest Med. 1985;6(2):219-36.
[2] Sieber F, Spivak JL, Sutcliffe AM. Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. Proc Natl Acad Sci U S A. 1984;81(23):7584-7.
[3] Khe?fets LB, Abalakin BA. Isolation of human blood elements in the verografin-fikoll density gradient. Lab Delo. 1973;10:579-81.
[4] Chernitskiy E. A., Vorobey A. V. Photosensitized damage to biological membranes. Mol. mechanisms biol. action. radiation. Nauka, 1988: 102-111.
[5] Kubasova T, K?teles GJ, Varga LP. Surface alterations of mammalian cells upon ionizing radiation as detected by lectin-binding technique. II. Binding of concanavalin A by human blood cells X-irradiated in vitro. Int J Radiat Biol Relat Stud Phys Chem Med. 1981;40(2):187-94.
[6] Shukanova NA, Lobanok ES, Vorobey AV. Change of structural and functional parameters of the membranes of thymocytes in irradiated animals. I scientific materials. Pract. conf. Minsk, 1989: 164-168.
[7] Vorobey AV, Vadetskaya TN, Yegorova GD, Chernitskiy YeA. Tetrasulfofenilporfinom photodamage sensitized erythrocyte membranes. Vestsi BSSR, Ser. biyal. Navuka. 1985;4: 103-5.
[8] Nicolson GL, Poste G. The cancer cell: dynamic aspects and modifications in cell-surface organization (first of two parts). N Engl J Med. 1976;295(4):197-203.
[9] Loor F. Plasma membrane and cell cortex interactions in lymphocyte functions. Adv Immunol. 1980;30:1-120.
[10] Patarroyo M, Gahmberg CG. Phorbol 12,13-dibutyrate enhances lateral redistribution of membrane glycoproteins in human blood lymphocytes. Eur J Immunol. 1984;14(9):781-7.
[11] Fidelus RK. The generation of oxygen radicals: a positive signal for lymphocyte activation. Cell Immunol. 1988;113(1):175-82.
[12] Timoshenko AV, Cherenkevich SN. Inhibitory effect of active oxygen scavengers on the endocytosis of concanavalin A by lymphocytes. Biofizika. 1987;32(3):434-7.
[13] Timoshenko AV, Cherenkevich SN, Samal' AB. Role of reactive oxygen species in a concanavalin A-induced lymphocyte agglutination. Herald BSU. Chem. biol. and geographer. 1986; 3: 47-50