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Structure and Function of Biopolymers
Determination of temperatures of structural-phase reconstructions in model and biomembranes from spectral characteristics of merocyaninc 540
- Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
14, Eugene Pottier Str., Kyiv, Ukraine, 03057
Absorption and fluorescence spectra of cyanine dye merocyanine 540 (M540) in organic solvents, liposomes from different classes of lipids, in rat liver microsomes and erythrocytes were studied. It was shown that together with the known way of determination of temperatures of phase-transitions Tn from absorption spectra of M540, determination of Tn from quantum yield of monomers was possible; at the same time the method proposed was more simple. Comparison of both methods was carried out.
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