Biopolym. Cell. 1993; 9(3):23-27.
http://dx.doi.org/10.7124/bc.000356
Structure and Function of Biopolymers
Affinity sorption analysis of the structure of poly(G)·poly(C) complex obtained by means of template-dependent synthesis
1Surzhik M. A., 1Timkovsky A. L.
  1. B. P. Konstantinov Petersburg Nuclear Physics Institute
    Gatchina, Leningrad district, Russian Federation, 188300

Abstract

The analysis of the structure of poly(G)·poly (C) complexes obtained by the enzymatic synthesis on poly(C) template was carried out using the method of affinity sorption. This latter was used also for the purification of the synthesized so far complexes from the redundant template as well as for the investigation of the structure of poly(G) ·poly(C) complexes obtained by the «traditional» mixing of complementary homopo-lynucleotides. It was shown that the degree of template transcription depends on the quality of RNA polymerase and the incompleted transcription is connected with the inhibition of the elongation of poly(G) chain.
Full text: (PDF, in English)

References

[1] Surzhyk MF, Bil'ner LM, Kachurin AM, Timkovskiy AL. Matrix synthesis of the poly (T)-poly (C) with a DNA-dependent polymerase RVC Escherichia coli. Study of physico-chemical properties and biological activity of the reaction product. L., (Preprint. USSR. Inst nuclei physics; 1555). 1989: 11-18.
[2] Vil'ner LM, Timkovskiy AL, Tikhomirova-Sidorova NS. Biological activity and structural features of the interfefone inducer polynucleotides. Results of science and technology-M. VINITI (Ser. Virusologiya; vol. 7). Itogi nauki i tekhniki. M. : VINITI, 1977-S. 114-159.
[3] Glazunov EA. Chernaenko VM. New method enzymes immobilization on silicate media. Directed transport and immobilization of biologically active preparations for clinical practice. Kiev. Naukova Dumka, 1984: 11-12.
[4] Gilmor C. Chromatine transcrition. In. Transcription and translation-a practical approach: Edited by B D Hames and S J Higgins. IRL Press, Oxford. 1984. 328 p.
[5] TimkovskiÄ­ AL, Aksenov OA, Bresler SE, Kogan EM, Smorodintsev AA. Molecular weight characteristics of a polyriboguanylic-polyribocytidylic acid complex and its relation to antiviral and interferon-inducing activity. Vopr Virusol. 1973;18(3):350-5.
[6] Brabec V, Timkovsky AL. Electrochemistry of double-stranded complexes of synthetic polyribonucleotides having interferonogenic and antiviral activity. Gen Physiol Biophys. 1983;2(6):487-97.
[7] Surzhik MA, Dyatlova NP, Glazunov EA. et al. Stability of natural and synthetic polyribonucleotide interferon inducers ribonuclease to human blood. Antibiot Khimioter. 1992; 37(1):38-45.
[8] Colby C, Stollar BD, Simon MI. Interferon induction: DNA-RNA hybrid or double stranded RNA? Nat New Biol. 1971;229(6):172-4.
[9] Niyogi SK, Stevens A. Studies of the ribonucleic acid polymerase from Escherichia coli. 3. Studies with synthetic polyribonucleotides as templates. J Biol Chem. 1965;240:2587-92.