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Viruses and Cell
Nonradioactive hybridization test of Plasmodium falciparum infection of humans
- Institute of Cytology and Genetics, Siberian Branch of the RAS
10, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090
- E .I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine
20, M. Pirogov Str., Moscow, Russian Federation, 119435
Nonradioactive molecular hybridization test on Plasmodium falciparum infection in human peripheral blood is described. The DNA immobilization both on nylon and nitrocellulose membranes is carry out by UV irradiation. Nonradioactive biotinilated probe is prepared according to original method based on chemical modification of doublest-randed DNA. The correlation coefficient between microscopic examination data and hybridization data is 0.96 for infection density more then 25 parasites by 1000 leukocytes.
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