Biopolym. Cell. 1993; 9(1):19-22.
Structure and Function of Biopolymers
Protein biosynthesis in cell-free systems from pig myocardium under anoxia
1Kashauskas A. P., 1Ivanov L. L., 1Lukoshevichius L. J., 1Rodovichius H. A., 1Kondrotas D. Z.
  1. Kaunas Medical Academy
    Mizkevicha Str. 9, Kaunas, Lithuania, 44307

Abstract

Influence of anoxia on protein biosynthesis in cell-free system from pig myocardium was studied. The rate and level of [14C]-leucine incorporation into translational product were lower in cell-free system obtained from pig myocardium after anoxia than in system from control myocardium. Fluorography of electrophoregram of radioactive proteins synthesized in cell-free system has revealed a sharp decrease in the level of radioactive amino acids incorporation into total translational product 90 min after anoxia. The spectrum of proteins synthesized under these conditions did not change. The decrease in protein biosynthesis 20 min after anoxia is shown to depend on the alterations in cytosol, whereas 90 min after anoxia this phenomenon is related also to the changes in the ribosomal fraction.

References

[1] Jefferson LS, Wolpert EB, Giger KE, Morgan HE. Regulation of protein synthesis in heart muscle. 3. Effect of anoxia on protein synthesis. J Biol Chem. 1971;246(7):2171-8.
[2] Cobbe SM, Poole-Wilson PA. Tissue acidosis in myocardial hypoxia. J Mol Cell Cardiol. 1980;12(8):761-70.
[3] MacLennan PA, Rennie MJ. Effects of ischaemia, blood loss and reperfusion on rat muscle protein synthesis, metabolite concentrations and polyribosome profiles in vivo. Biochem J. 1989;260(1):195-200.
[4] Kashauskas AP, Tamuliavichius AA, Lukoshiavichius LIu, Ivanov LL, Prashkiavichius AK. Biological activity of tRNA and aminoacyl-tRNA-synthetases from the swine myocardium in anoxia and subsequent reoxygenation. Vopr Med Khim. 1988;34(2):84-6.
[5] Wettstein FO, Staehelin T, Noll H. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome. Nature. 1963;197:430-5.
[6] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[7] Schreiber SS, Oratz M, Rothschild MA. Post ischemic reperfusion and anoxic perfusion in the isolated heart: alteration in distribution of radionuclides and in protein synthesis. J Physiol (Paris). 1980;76(7):777-84.
[8] White FP, White SR. Isoproterenol induced myocardial necrosis is associated with stress protein synthesis in rat heart and thoracic aorta. Cardiovasc Res. 1986;20(7):512-5.
[9] Currie RW. Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts. J Mol Cell Cardiol. 1987;19(8):795-808.
[10] Havre PA, Hammond GL. Isolation of a translation-inhibiting peptide from myocardium. Am J Physiol. 1988;255(5 Pt 2):H1024-31.
[11] Prashkevichius A, Maskoliunas R, Stapulionis R. et al. Protein biosynthesis machinery under myocardial ischemia. Exp Biol. 1990; 1:47-55.