Biopolym. Cell. 1992; 8(4):43-48.
Heterogeneity of the line of human bone marrow stromal precursor cells
- Institute of Traumatology and Orthopedics MAS of Ukraine
27, Vorovskogo Str., Ukraine, Kyiv, 01601
It has been studied the characteristics of the colony forming units of fibroblast (CFUf) by cultivation with rabbit feeder. The line of stromal precursor cells of human bone marrow is heterogenic as to its proliferative potential that is manifested with various attitude of CFUf to feeder. There are 2 fractions of CFUf: feeder dependent, forming the colonies in culture, forming the colonies in culture only with the addition of feeder and feeder independent forming the colonies without feeder. One part of CFUf forming in ordinary condition clusters, makes some additional divisions and grows to a colony, the second part of CFUf has no ability to form colonies in such condition. The heterogeneity of CFUf causes on the one hand the properties of CEUf itself, that is their proliferative potential, and on the other hand depends on the influence of microenvironment, creating by unadhesive fraction of bone marrow cells.
 Astakhov VS Properties stromal progenitor cells in the bone marrow of the orthopedic-trauma pathology and prospects for their clinical use. Author. dis. ... Dr. med. nauk. Kyiv, 1987: 32 p.
 Friedenstein AJ, Lalykina KS. Induction of bone tissue and osteogenic progenitor cells. M.: Meditsina, 1973. 223 p.
 Friedenstein AJ, Luria EA Cellular basis of hematopoietic microenvironment. M: Meditsina. 1980. 216 p.
 Astakhova VS. Comparative evaluation of xenofeeders in cloning of stromal fibroblasts of human bone marrow. Biull Eksp Biol Med. 1982;94(10):111-3.
 Zaritski? AIu, Kazhdan IIa, Kulik MA, Lisovskaia VA, Iartseva NM. Colony-forming ability of bone marrow fibroblast precursor cells in chronic myeloid leukemia patients. Ter Arkh. 1982;54(8):120-3.
 Kolesnikova AI, Khoptynskaia SK, Ba?sogolov GD, Shishkin IP. Various factors influencing the colony forming ability of human bone marrow cells. Ter Arkh. 1982;54(8):104-8.
 Kolesnikova AI, Khoptynskaia SK, Zherbin EA, Vorob'ev AI, Domracheva EV. Characteristics of the stromal bone marrow precursor cells in patients without blood system changes. Probl Gematol Pereliv Krovi. 1978;23(11):35-8.
 Castro-Malaspina H, Ebell W, Wang S. Human bone marrow fibroblast colony-forming units (CFU-F). Prog Clin Biol Res. 1984;154:209-36. Review.
 Nagao T, Yamauchi K, Komatsuda M. Serial in vitro bone marrow fibroblast culture in human leukemia. Blood. 1983;61(3):589-92.
 Zipori D, Reichman N, Arcavi L, Shtalrid M, Berrebi A, Resnitzky P. In vitro functions of stromal cells from human and mouse bone marrow. Exp Hematol. 1985;13(7):603-9.
 Kulagina NN, Luriia EA, Astakhova VS, Genkina EN. Method of cloning human bone marrow stromal cells. Probl Gematol Pereliv Krovi. 1981;26(11):39-41.