In vivo comparison of the regulatory ability of L10 ribosomal proteins with different primary structures

Zhyvoloup, A. N.; Kroupskaya, I. V.; Woodmaska, M. I.; Paton, E. B.

doi:10.7124/bc.000324

Biopolym. Cell. 1992; 8(3):39-42.

http://dx.doi.org/10.7124/bc.000324

Genome and Its Regulation

In vivo comparison of the regulatory ability of L10 ribosomal proteins with different primary structures

1Zhyvoloup A. N., 1Kroupskaya I. V., 1Woodmaska M. I., 1Paton E. B.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A system for estimation and comparison of the regulatory ability of L10 ribosomal proteins was elaborated. pAZ102 plasmid, carrying the reporter rplJ'-lacZ gene was cointroduced into Echerichia coli cells together with the L10-producing plasmids. The regulatory ability of the assayed L10 proteins was estimated by their efficient inhibition of expression of the reporter gene

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