Biopolym. Cell. 1992; 8(3):23-29.
http://dx.doi.org/10.7124/bc.000320
Structure and Function of Biopolymers
Detection of pUC19-homologous repeated sequences into genome of several species of higher plants
1Patskovsky Yu. V., 1Gaiduk V. V., 1Veselovsky O. V., 2Zubko E. I., 2Pasternak T. P., 1Yurkevitch L. N., 2Mashtaler S. G., 1Potopalsky A. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

With Southern blot hybridization into a nuclear DNA of the several species of higher plants (Secale cereale, Zea mays, Trilicum qestivum, Solanum nigrum, S. tuberosum, Atropa belladonna, Licopersicon esculentum) the pUC19-homologous repeated DNA sequences are detected. In the genomic DNA of S. cereale and A. belladonna its are clustered. The difference to the pUC-repeats organization and to their copies number between several species and several lines (or generations – S. cereale) of each species is shown. The possibility of using of the pUC-homologous sequences as a marker for somatic hybridization is demonstrated. The possible correlation of the showed facts with plant genome instability is discussed.
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