Biopolym. Cell. 1991; 7(6):96-99.
Gene-Engineering Biotechnology
The influence of the regulatory mutation in the rib1 locus on the riboflavin biosynthesis in Pichia guilliermondii
1Shavlovsky G. M., 1Stenchuk N. N., 1Kshanovskaya B. V.
  1. Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005


The rib1-86 mutant of P. guilliermondii in which riboflavin auxotrophy is suppressed by mutation rib81, but not by rib80 was selected. Double ribl-86 rib81 mutant is characterized by low GTP-cyclohydrolase activity. The enzyme synthesis is faintly regulated by iron. It is supposed that ribl-86 mutation is localized in the promoter region of RIB1 gene which codes for GTP-cyclohydrolase.


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