Biopolym. Cell. 1991; 7(6):96-99.
Gene-Engineering Biotechnology
The influence of the regulatory mutation in the rib1 locus on the riboflavin biosynthesis in Pichia guilliermondii
1Shavlovsky G. M., 1Stenchuk N. N., 1Kshanovskaya B. V.
  1. Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005

Abstract

The rib1-86 mutant of P. guilliermondii in which riboflavin auxotrophy is suppressed by mutation rib81, but not by rib80 was selected. Double ribl-86 rib81 mutant is characterized by low GTP-cyclohydrolase activity. The enzyme synthesis is faintly regulated by iron. It is supposed that ribl-86 mutation is localized in the promoter region of RIB1 gene which codes for GTP-cyclohydrolase.

References

[1] Shavlovskiy GM, Fedorovich DV, Logvinenko EM, Koltun LV. The isolation and characterization of Pichia guilliermondii strains producing riboflavin and having the regulator mutation rib80 (ribR). Mikrobiologiia. 1985;54(6):919-26.
[2] Shavlovski? GM, Babiak LIa, Sibirny? AA, Logvinenko EM. Genetic control of riboflavin biosynthesis in Pichia guilliermondii yeasts. The detection of a new regulator gene RIB81. Genetika. 1985;21(3):368-74.
[3] Shavlovskiy GM, Koltun LV, Kshanovskaya BV et al. Regulation of riboflavin biosynthesis by positive control elements in the yeast Pichia guilliermondii. Genetika. 1987. 25, 2:250-258.
[4] Guarente L. Regulatory proteins in yeast. Annu Rev Genet. 1987;21:425-52. Review.
[5] Shavlovski? GM, Sibirny? AA, Kshanovskaia BV, Koltun LV, Logvinenko EM. Genetic classification of riboflavin-dependent mutants of Pichia guilliermondii yeasts. Genetika. 1979;15(9):1561-8.
[6] Waring WS. Werkman CH. Grouth of bacteria in iron-free medium. Arch Biochem. 1943; 4(2):303-10.
[7] Logvinenko EM, Shavlovski? GM, Zakal'ski? AE, Zakhodylo IV. Effect of iron, actinomycin D and cycloheximide on the GTP-cyclohydrolase synthesis in flavinogenic yeasts. Biokhimiia. 1982;47(1):28-31.
[8] Logvinenko EM, Shavlovski? GM, Trach VM, Sibirny? VA. Role of flavins in regulating riboflavin synthetase synthesis in Pichia guilliermondii and Candida utilis. Mikrobiologiia. 1973;42(6):1008-14.
[9] Mironov VN, Chikindas ML, Kraev AS, Stepanov AI, Skriabin KG. The operon organization of genes for riboflavin biosynthesis in Bacillus subtilis. Dokl Akad Nauk SSSR. 1990;312(1):237-40.
[10] Zakal'ski? AE, Zlochevski? ML, Stasiv IuZ, Logvinenko EM, Beburov MIu, Shavlovski? GM. Cloning of the RIB1 gene coding for the enzyme of the first stage of flavinogenesis in the yeast Pichia guilliermondi, GTP cyclohydrolase, in Escherichia coli cells. Genetika. 1990;26(4):614-20.