Biopolym. Cell. 1991; 7(6):5-15.
Structure and Function of Biopolymers
Investigation of interaction of proflavine with deoxytetraribonucleoside triphosphate 5'-d(ApCpGpT) by the method of 1H-NMR spectroscopy
- Sevastopol Instrument Engineering Institute
33, Universitetskaya Str., Sevastopol, Ukraine, 99053
- Birkbeck, University of London
Malet Str., Bloomsbury, London WC1E 7HX, UK
Complex formation between acridine dye proflavine and self-complementary deoxytetra-ribonucleoside triphosphate 5'-d(ApCpGpT) in water-salt solution has been studied by the method of one-dimensional and two-dimensional 1H-NMR spectroscopy (500 MHz). Two-dimensional homonuclear 1H-NMR spectroscopy (2D-COSY and 2D-NOESY) was used for complete assignments of proton signals of molecules in solution and for qualitative analysis of the nature of interactions of proflavine with tetranucleotide. Concentration dependences of proton chemical shifts of the molecules have been measured at 293 K. Analysis of relative content of different types of complexes has been made and special features of dynamic equilibrium have been revealed as a function of correlation of dye and tetranucleotide concentrations. Based on the obtained results a conclusion has been made about preferential intercalation of proflavine to CG-site of tetranucleotide in duplex form. The most favourable structure of 1 : 2 complex of dye with tetranucleotide has been constructed using calculated values of induced chemical shifts of proflavine protons and 2D-NOESY spectra.
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