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Chloroplast DNA topoisomerase I from pea leaves
- Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Chloroplasts of pea leaves were isolated by step gradient isoosmotic percoll, chloroplast DNA-topoisomerase. was isolated from them by fractionation in two-phase system ammonium sulfate/polyethylenglycol-6000 with the following purification by using column chromatography. The some properties of DNA-topoisomerase was searched. The dependence of relax activity of the enzyme from Mg2+ ions, its blocking by ethydium bromide, the absence of relax activity stimulation by polyamines: spermidine, spermin, cadaverine and the character of distribution of the topoisomers in the gels allow us to characterise the topoisomerase as prokaryotic type I.
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