Biopolym. Cell. 1991; 7(4):95-97.
Cell Biology
pYAC-GN, a yeast artificial chromosome vector which codes GUS and APH(3')II reporter genes for plant cells
1Klimyuk V. I.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The vector pYAC-GN has been constructed in order to be able to introduce YAC clones into plant cells for functional analysis of large fragments of DNA. To facilitate the selection of plant cells which have taken up YACs, the pYAC-4 was modified by insertion of GUS and APH(3')II reporter genes under 35S promoter of CaMV into the Clal and Sail sites respectively. The polylinker contaning NotI, Clal and Sail unique restriction sites was also introduced into EcoRI site of SUP4.


[1] Burke DT, Carle GF, Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987;236(4803):806-12.
[2] Pachnis V, Pevny L, Rothstein R, Costantini F. Transfer of a yeast artificial chromosome carrying human DNA from Saccharomyces cerevisiae into mammalian cells. Proc Natl Acad Sci U S A. 1990;87(13):5109-13.
[3] Pavan WJ, Hieter P, Reeves RH. Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome. Mol Cell Biol. 1990;10(8):4163-9.
[4] Ganal MW, Tanksley SD. Analysis of tomato DNA by pulsed field gel electrophoresis. Plant Mol Biol Rep. 1989;7(1):17–28.