Biopolym. Cell. 1991; 7(4):54-60.
Cell Biology
Isolation of asymmetric somatic hybrids of medicago species
1Kuchuk N. V., 1Shakhovski A. M., 1Voronin V. V., 1Gleba Yu. Yu.
- Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
Interspecific somatic hybrids of Medicago have been obtained by «gamma-fusion». Kanamycin resistant plants of M. varia and kanamycin resistant callus line of M. lupulina were used as donors. M. sativa and M. borialis were used as recipients. After fusion of gamma-irradiated (300 Gy) mesophyll protoplasts of M. varia with those of M. sativa, 13 kanamycin resistant hybrid clones were selected. We could induce the formation plant regenerants from one of the clones. Chromosome number of regenerated plants was
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