Biopolym. Cell. 1991; 7(4):45-50.
Cell Biology
Mitochondrial genome of genus Nicotiana
1Cherep N. N., 1Komarnitsky I. K.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Mitochondrial DNA (mt DNA) restriction fragment patterns 38 Nicotiana species were used to study phylogenetic relationship. Results indicated that the N. rustica mt DNA was inherited from N. paniculata. Conservation in organization between mt DNA of N. rustica and N. paniculata, N. acuminata and N. attenuata, N. palmeri and N. trigonophylla provide evidence that the mt genome for these pairs of species is evolutionary stable. On the base of the mt DNA analysis the species of Suaveolens section can be Dividet into two groups.


[1] Psareva EN. On the genus Nikotsiana. Proc. VITIM. 1963; Iss 153:10-153.
[2] Chen K, Jahal S, Wildman SD. Role of chloroplast and nuclear DNA genes during evolution of fraction 1 protein. Genet, and biogenesis of chloroplast and mitochondria. Amsterdam: Elsevier, 1976:3-11.
[3] Kung SD, Sakano K, Gray JC, Wildman SG. The evolution of fraction i protein during the origin of a new species of Nicotiana. J Mol Evol. 1975;7(1):59-64.
[4] Wildman S. G. Polypeptide composition of Rubisko as an aid in studies of plant phytogeny. Protein and Nucl. Acids in Plant System. Berlin; Springer, 1983;182-90.
[5] Grayburn WS, Bendich AJ. Variable abundance of a mitochondrial DNA fragment in cultured tobacco cells. Curr Genet. 1987;12(4):257-61.
[6] Sparks RB, Dale RMK. Characterization of 3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cells. Mol Gen Genet. 1980;180(2):351–5.
[7] Dale RMK. The structure and organization of the mitochondrial genome of plant tissue culture cells. J Cell Biol. 1980; 87(2):185.
[8] Dale RM, Wu M, Kiernan MC. Analysis of four tobacco mitochondrial DNA size classes. Nucleic Acids Res. 1983;11(6):1673-85.
[9] Manna F, Del Giudice L, Massardo DR et al. The mitochondrial genome of Nicotiana plumbaginifolia. Curr Genet. 1985; 9(5):411-5.
[10] Davydenko OG, Komarnicki IK, Samoilov AM. Gleba YuYu. Intraspecific variation in the mitochondrial genome Triticum eastivum L. Doklady Akad Nauk Ukr SSR. Ser B. 1989; 4:58-61.
[11] Fontarnau A, Hern?ndez-Yago J. Characterization of mitochondrial DNA in citrus. Plant Physiol. 1982;70(6):1678-82.
[12] Ichikawa H, Tanno-Suenaga L, Imamura J. Mitochondrial genome diversity among cultivars of daucus carota (ssp. sativus) and their wild relatives. Theor Appl Genet. 1989;77(1):39–43.
[13] Komarnitsky IK, Samoylov AM, Red'ko VV, Peretyayko VG, Gleba YY. Intraspecific diversity of sugar beet (Beta vulgaris) mitochondrial DNA. Theor Appl Genet. 1990;80(2):253-7.
[14] McClean PE, Hanson MR. Mitochondrial DNA Sequence Divergence among Lycopersicon and Related Solanum Species. Genetics. 1986;112(3):649-67.
[15] Kemble RJ, Gunn RE, Flavell RB. Mitochondrial DNA variation in races of maize indigenous to Mexico. Theor Appl Genet. 1983;65(2):129-44.
[16] Negruk VI, Kaushik NK. Structural variations in Vicia faba mitochondrial genome. Theor Appl Genet. 1988;76(4):587-92.
[17] Ward BL, Anderson RS, Bendich AJ. The mitochondrial genome is large and variable in a family of plants (cucurbitaceae). Cell. 1981;25(3):793-803.
[18] Chowdhury MK, Smith RL. Mitochondrial DNA variation in pearl millet and related species. Theor Appl Genet. 1988;76(1):25-32.
[19] Berthou F, Mathieu C, Vedel F. hloroplast and mitochondrial DNA variation as indicator of phylogenetic relationships in the genus Coffea L. Theor Appl Genet. 1983;65(1):77-84.
[20] Wilson AJ, Chourey PS. A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources. Plant Cell Rep. 1984;3(6):237-9.
[21] Gray JC. Serological reactions of Fraction I proteins from interspecific hybrids in the genusNicotiana. Plant Systematics and Evolution. 1978;129(3):177–83.
[22] Komarnitsky IK, Samoylov AM, Gleba YuYu. Restriction mapping of chloroplast DNAs of five Nicotiana species. Biopolym Cell. 1989;5(1):66-72.
[23] Bland MM, Matzinger DF, Levings CS 3rd. Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species. Theor Appl Genet. 1985;69(5-6):535-41.
[24] Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS. Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet. 1984;69(1):1-14.
[25] Gray JC, Kung SD, Wildman SG. Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein. Nature. 1974;252(5480):226-7.
[26] Kung SD, Zhu YS, Shen GF. Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor Appl Genet. 1982;61(1):73-9.