Biopolym. Cell. 1991; 7(3):95-98.
Cell Biology
D2O inhibit microtubules assembly in vitro
1Sorochinsky B. V., 1Prohnevsky A. I.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The influence of the deuterium water different concentrations (25 – 75%) on the polymerization of bovine brain microtubules has been investigated. It was shown that D2O inhibited microtubules assembly in vitro.

References

[1] Lamprecht J, Schroeter D, Paweletz N. Mitosis arrested by deuterium oxide. Light microscopic, immunofluorescence and ultrastructural characterization. Eur J Cell Biol. 1990;51(2):303-12.
[2] Scharf SR, Rowning B, Wu M, Gerhart JC. Hyperdorsoanterior embryos from Xenopus eggs treated with D2O. Dev Biol. 1989;134(1):175-88.
[3] Sumitro SB, Sato H. The isotopic effects of D2O in developing sea urchin eggs. Cell Struct Funct. 1989;14(1):95-111.
[4] Zimmermann A, Keller HU, Cottier H. Heavy water (D2O)-induced shape changes, movements and F-actin redistribution in human neutrophil granulocytes. Eur J Cell Biol. 1988;47(2):320-6.
[5] Mita H, Yui Y, Yasueda H, Shida T. Effect of deuterium oxide on leukotriene C4 generation in immunologically stimulated human leukocytes. Int Arch Allergy Appl Immunol. 1988;85(4):422-7.
[6] Lin PS, Hefter K, Ho KC. Modification of membrane function, protein synthesis, and heat killing effect in cultured Chinese hamster cells by glycerol and D2O1. Cancer Res. 1984;44(12 Pt 1):5776-84.
[7] Leonard PJ, Mullins JM. D2O induced alterations of mitosis in PtK1 cells. Exp Cell Res. 1987;172(1):204-11.
[8] Fulton A. The Cytoskeleton: Cellular Architecture and Choreography? Chapman and Hall. 1984; 80 p.
[9] Shelanski ML, Gaskin F, Cantor CR. Microtubule assembly in the absence of added nucleotides. Proc Natl Acad Sci U S A. 1973;70(3):765-8.
[10] Bershadsky AD, Gelfand VI, Svitkina TM, Tint IS. Microtubules in mouse embryo fibroblasts extracted with Triton X-100. Cell Biol Int Rep. 1978;2(5):425-32.
[11] Greenberg CS, Craddock PR. Rapid single-step membrane protein assay. Clin Chem. 1982;28(7):1725-6.
[12] Sorochinski? BV, Prokhnevski? AI, Grodzinski? DM. simple method for isolating biologically active drugs taxol from tissues Taxus baccata. Chemistry of natural compounds;1990;5:702-3.
[13] Voter WA, Erickson HP. The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. J Biol Chem. 1984;259(16):10430-8.
[14] Prohnevsky AI, Sorochinskiy BV. Modification ethanol brain microtubule assembly. Neyrokhimiia. 1990; 9(1):96-100.