Biopolym. Cell. 1991; 7(1):101-107.
Gene-Engineering Biotechnology
Study of β-lactamase tem1 synthesis in various escherichia coli strains infected by the phage λ bla with amber-mutations in different regulatory genes
1Sorochynskaja T. V., 1Chernykh S. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The search of optimal conditions for phage-dependent supersynthesis of β-lactamase was undertaken. For this purpose the recombinant phage with alternatively orientated β-lactamase (bla) structure gene were obtained. It was stated, that phage λblaNN-, λblaQR derivates are obtained. The β-lactamase producing strain was selected. The maximal recovery of β-lactamase was obtained while Su0 E. coli W3101 strain was infected by λ-phage bla QR.

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