Biopolym. Cell. 1991; 7(1):82-84.
Structure and Function of Biopolymers
Investigation of cytochrome P-450 interaction with phospholipids using fluorescence spectroscopy
1Gorbenko G. P., 1Dyubko T. S., 1Nardid O. A., 1Moiseev V. A.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015


The technique of fluorescence quenching permits studying the influence of phospholipids on the structural state of cytochrome P-450. It is supposed that the depth of protein incorporation in the lipid matrix of model membrane decreases in the presence of acid phospholipids.


[1] Akhrem AA, Romanov SL, Karasev VP, Kisel' MA, Kiselev PA. Temperature quenching of cytochrome P-450 fluorescence in proteoliposomes with different physical states of the lipid bilayer. Biofizika. 1988;33(2):275-81.
[2] Miasoedova KN, Berndt P. Cytochrome P-450 in proteoliposomes: oligomeric structure of the LM2 isoform. Biokhimiia. 1989;54(9):1445-56.
[3] Uvarov VIu, Davydov RM, Timofeev VP. ESR-spectroscopy of cytochrome P-450 LM2 in the soluble and membrane-bound state. Biofizika. 1988;33(4):719-20.
[4] Kiselev PA, Garda G, Finch SA, Stir A, Gurinovich NA, Khatyleva SIu, Akhrem AA. Rotational diffusion of cytochrome P-450 in a model membrane of various phospholipid composition. Dokl Akad Nauk SSSR. 1989;307(2):473-6.
[5] Haugen DA, Coon MJ. Properties of electrophoretically homogeneous phenobarbital-inducible and beta-naphthoflavone-inducible forms of liver microsomal cytochrome P-450. J Biol Chem. 1976;251(24):7929-39.
[6] Lakowicz JR, Principles of Fluorescence Spectroscopy. Plenum Press, New York, London, 1983.
[7] Demchenko AP. Luminescence and protein structure dynamics. Kiev. Naukova Dumka, 1988. 280 p.
[8] Heinemann FS, Ozols J. The complete amino acid sequence of rabbit phenobarbital-induced liver microsomal cytochrome P-450. J Biol Chem. 1983;258(7):4195-201.
[9] Vernike D, Volodin YuYu, Uvarov VYu, Archakov AI. Effect of phospholipids and proteins on the conformational state of cytochrome P-450. Proc. conf. "Cytochrome P-450 and protection of the internal environment of man": Proc. of reports. Pushchino, 1985:35.